Journal: eLife
Article Title: Atlas of Fshr expression from novel reporter mice
doi: 10.7554/eLife.93413
Figure Lengend Snippet: Frozen sectioned liver tissue was stained for Col1a1 ( A ), CD31 ( B ), or KCNMA1 ( C ), followed by florescent imaging for Fshr-ZsGreen and each of these stained molecules simultaneously. In the section stained for Col1a1 ( A ), two representative areas are shown at magnifications of ×400 and ×1000 as indicated: (1) hepatic cells with a central vein (CV) ( a and c ) and (2) hepatic artery ( b and d ). In the section stained for CD31 ( B ), a representative area of hepatic cells with a CV is shown at lower ( a ) and higher ( b ) magnifications. In the section stained for KCNMA1, three representative areas are presented at two magnifications as follows: (1) hepatic cells with a CV ( a and b ); (2) hepatic cells with peripheral nerve fibers ( c and d ); and (3) small nerve fibers located around a vein ( e and f ).
Article Snippet: Anti-KCNMA1/BK channel Ab , Bioss , bs-4775R , 1:200.
Techniques: Staining, Imaging